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91.
92.
Akiko Watanabe Keiichi Ohata Tsuyoshi Oikawa Takeshi Sugaya Masao Miyazaki Hiroshi Satoh Masaaki Katayama 《Canadian journal of veterinary research》2021,85(2):156
Urinary liver-type fatty acid-binding protein (uL-FABP) is a clinically useful biomarker for monitoring chronic kidney disease (CKD) in humans. However, long-term monitoring of uL-FABP in CKD cats has not been reported. The objective of this preliminary study was to investigate whether the urinary excretion of L-FABP could predict the deterioration of renal function in 2 CKD model cats. Urinary liver-type fatty acid-binding protein (uL-FABP) increased before standard renal biomarkers, including serum creatinine, blood urea nitrogen, and symmetric dimethylarginine, in 1 cat with deteriorating renal function, but remained low and relatively stable in another cat with stable renal function. Our results suggest that uL-FABP is a potential clinical biomarker for predicting the progression of CKD in cats, as it is in humans. 相似文献
93.
Impact of fermented milk on human health: Cholesterol-lowering and immunomodulatory properties of fermented milk 总被引:3,自引:0,他引:3
Akiyoshi HOSONO Hajime OTANI Hisako YASUI Masaaki WATANUKI 《Animal Science Journal》2002,73(4):241-256
In this review, we focus on the cholesterol-lowering activity of Lactobacillus gasseri , the immunomodulatory function of casein phosphopeptides (CPPs) and lactic acid bacteria as starters for fermented milk, and the probability and difficulty of creating immunomodulatory fermented milk as a 'food for specified health use'. Among the starter bacteria used for the preparation of fermented milk, L. gasseri SBT0270 could exert a hypocholesterolemic effect in rats fed a diet high in cholesterol through deconjugation of bile salts. On the other hand, CPPs, having a tripeptide sequence consisting of two phosphoserine residues at N- and C-terminal positions, stimulated lymphocyte proliferation and IgA formation via the increase of interleukin-5 and interleukin-6 produced by type 2 helper T cells. The oral ingestion of CPPs enhanced the level of antigen-specific and total intestinal IgA. Moreover, one strain of bifidobacteria ( Bifidobacterium breve YIT4064) and one strain of Lactobacilli ( Lactobacillus casei strain Shirota), used as a starter for fermented milk, activated the humoral and cellular immune system, respectively, and prevented various diseases. It is important to elucidate what components modulate and what mechanisms are responsible for the physiological function of fermented milk in order for it to be accepted as a 'food for specified health use' claiming immunomodulatory activity. 相似文献
94.
95.
Ikemoto T Suzuki M Onoe H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(6):733-738
Skeletal-type neuroendocrine-specific protein like 1 (sk-NSPL1) has been demonstrated to be physiologically important in regulating the membrane translocation of glucose transporter 4 (GLUT4) in skeletal muscles. We investigated the levels of phosphorylation in proteins that are thought to be involved in exercise in wild-type and sk-NSPL1-deficient muscles with specific antibodies and phosphate-metal affinity chromatography resin (p-resin). In both normal skeletal muscle and sk-NSPL1-deficient muscle, adenosine monophosphate (AMP)-dependent kinase (AMPK) and acetyl-CoA carboxylase (ACC) were phosphorylated and adsorbed onto p-resin at high levels after exercise. On the other hand, the effect of 5-aminoimidazole-4-carboxyamide ribonucleoside (AICAR), which is an activator of AMPK, in blood glucose was greatly diminished in mutant mice. P-resin adsorbed sk-NSPL1 in the membrane fraction from wild-type muscle after exercise and AICAR administration. Isolated sk-NSPL1 from wild-type also had increased adsorption onto p-resin after treatment with Ca(2+) and adenosine triphosphate (ATP). After long-term incubation of sk-NSPL1-containing membrane without ATP, sk-NSPL1 adsorption onto anion-exchange resin was drastically reduced. These results suggest that the function of sk-NSPL1 is regulated by a [Ca(2+)](i)- and AMPK-mediated pathway under exercise, and support the hypothesis that sk-NSPL1 is an important factor in the downstream of the exercise-dependent pathway in GLUT4 translocation. 相似文献
96.
Sakai H Yonemaru K Takeda M Niimi K Murakami M Hirata A Yanai T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(6):801-803
An 11-year-old male Labrador retriever presented with chronic oliguria. Ultrasonography findings revealed a protruding mass at the neck of the urinary bladder. A cystotomy was performed, and the mass was removed by ligation with surgical sutures. Histopathological examination revealed conspicuous foci with a variable number of ganglion cells in the tumor and abundant interwoven bundles of schwannian cells with fine fibers. The ganglion cells were positive for neuron-specific enolase and neurofilament. The schwannian cells were positive for vimentin, S-100 protein, and glial fibrillary acidic protein. Thus, according to the classification of tumor with neuronal cell differentiation, the urinary tumor was diagnosed as a ganglioneuroma. 相似文献
97.
Trang VT Takeuchi H Kudo H Katsuno S Shimamura T Kashiwagi T Son VH Sugiura T Ukeda H 《Journal of agricultural and food chemistry》2011,59(16):8953-8960
In this study, antimicrobial activity of aminoreductone (AR), a product formed during the initial stage of the Maillard reaction, against methicillin-resistant Staphylococcus aureus (MRSA) was evaluated. The significant growth inhibition of all 51 MRSA isolates irrespective of drug susceptibility by AR was observed. The minimum inhibitory concentration (MIC) of AR ranged from 13 to 26 mM. The bactericidal activity of AR was evaluated by a killing assay with multiples of MIC, and it was recognized to depend on its dose. The combined effects of AR and antibiotics frequently used for the treatment of infections caused by Gram-positive and Gram-negative bacteria, such as amikacin (AN), ciprofloxacin, imipenem and levofloxacin, were examined. As a result, AR did not interfere with these antibiotic activities against 12 MRSA isolates selected and showed the advanced effect of growth inhibition in combination with antibiotics. Moreover, the inhibitory effects of AR were similar to those of AN, an antibiotic with known adverse effects, some serious. These findings show that AR is a naturally formed antimicrobial agent present in thermally processed foods with potential health benefits in medical practice. 相似文献
98.
Yu Sasada Khin Thawda Win Ryoko Nonaka Aye Thida Win Koki Toyota Takashi Motobayashi Masaaki Hosomi Chen Dingjiang Jun Lu 《Biology and Fertility of Soils》2011,47(8):949-956
We have examined the effects of different types of slurry on CH4 and N2O emissions, Zn and Cu contents of rice, and nitrate content of the drainage water. The experiment included four treatments: (1) anaerobically digested cattle slurry (ADCS), (2) ADCS filtered to remove the coarse organic matter fraction, (3) anaerobically digested pig slurry (ADPS), and (4) chemical fertilizer (CF). The application rate was 30?g?NH4?CN?m?2. Different amounts of C were incorporated with fertilization: 725?g?C?m?2 in ADCS, 352?g?m?2 in filtered ADCS, and 75?g?m?2 in ADPS. The average CH4 emissions during a growing period were 304, 359, 452, and 579?mg?m?2?day?1 in the CF, ADPS, filtered ADCS, and ADCS treatments, respectively. The CH4 emission was significantly higher in ADCS than in CF and ADPS. Negligible N2O emissions were observed during the growing period. Comparable concentrations of Zn and Cu were observed in the rice grain among the treatments. In contrast, their concentrations in the stems and leaves were significantly higher in ADPS than in CF treated rice, although the values were lower than the upper limit of feed additives. Nitrate concentrations in the drainage water were consistently low (0.5?mg?N?L?1). The present study suggested that ADPS, containing a lower amount of C than ADCS, might be an organic fertilizer in paddy field with comparable environmental impacts to chemical fertilizers (CF), but long-term field studies are needed to better understand the effects of these organic fertilizers. 相似文献
99.
Crude extracts of tentacles of two polychaetous annelids completely inhibit growth of Erlich ascites tumor in 60 to 100 percent of treated mice. Dialyzed extracts of one of these annelids, Lanice conchilega, show activity in the retentate after pronase digestion, suggesting that antitumor activity is associated with a nonprotein component of the crude tentacle extract. 相似文献
100.
The effects of extracellular ions on the acquisition and maintenance of the potential for motility in the spermatozoa of the ayu, Plecoglossus altivelis Temminck et Schlegel (Osmeridae), were examined. Testicular spermatozoa and milt spermatozoa were obtained from fully matured males and diluted with buffered solution (BS, 20 mM HEPES–NaOH, pH 7.5). Testicular spermatozoa showed a significantly low rate of motility (0.8 ± 0.4%), whereas milt spermatozoa showed a high rate (89.4 ± 2.1%). The spermatozoa were incubated with various isotonic media for 2 h, diluted with BS, and changes in the rates of motility were then compared. When incubated for 2 h with artificial seminal plasma (ASP), corresponding in terms of ionic constituents to seminal plasma buffered at pH 8.0, both spermatozoa showed a high rate of motility. Testicular spermatozoa acquired and milt spermatozoa maintained the potential for motility in response to the HCO3– ion concentrations (between 0 and 20 mM) in the ASP. The differences in the pH of the ASP had a significant effect on the acquisition and maintenance of the potential for motility, and spermatozoa showed the highest rate of motility with the ASP at pH 8.0 and 8.5. These results suggest that the quality of milt in the ayu can be regulated by controlling the concentration of bicarbonate and the pH of the incubating media. 相似文献